Sequence-Based Analyses of Biosynthesis Rate Limiting Factors in Wheat Germ Cell-Free System
نویسندگان
چکیده
Kenta Nakai [email protected] 1 Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan 2 Plant Functional Genomics Research Team, Functional Genomics Research Group, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 3 RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 4 Department of Applied Chemistry, Faculty of Engineering, and Venture Business Laboratory, Ehime University, Matsuyama 790-8577, Japan
منابع مشابه
Inhibition of cell-free protein synthesis by low-molecular-weight nuclear polyadenylate-containing ribonucleic acid species isolated from the lactating guinea pig.
1. Poly(A)-containing RNA was isolated from the nuclei of mammary gland, liver and brain of lactating guinea pigs. 2. Total nuclear poly(A)-containing RNA from mammary gland inhibited mRNA-directed protein synthesis by a wheat-germ cell-free system. It also inhibited the endogenous activity of the wheat-germ and other cell-free systems. It did not inhibit a wheat-germ cell-free system directed ...
متن کاملA systematic approach for testing expression of human full-length proteins in cell-free expression systems
BACKGROUND The growing field of proteomics and systems biology is resulting in an ever increasing demand for purified recombinant proteins for structural and functional studies. Here, we show a systematic approach to successfully express a full-length protein of interest by using cell-free and cell-based expression systems. RESULTS In a pre-screen, we evaluated the expression of 960 human ful...
متن کاملClassification of binding preferences for 1000 novel transcription factors synthesized using a wheat germ cell-free translation system and Biacore analysis
Label-free interaction analysis using BiacoreTM systems, combined with a highly efficient cell-free translation system provides a powerful approach to establish the targets of novel transcription factors. Interactions between 1000 proteins and 53 DNA oligonucleotide sequences were performed directly from in vitro translation microplates, greatly simplifying DNA binding analysis compared to trad...
متن کاملEffect of Different Concentrations of Forskolin Along with Mature Granulosa Cell Co-Culturing on Mouse Embryonic Stem Cell Differentiation into Germ-Like Cells
Background: Germ cell development processes are influenced by soluble factors and intercellular signaling events between them and the neighboring somatic cells. More insight into the molecular biology of the germ cell development from embryonic stem (ES) cells and investigation of appropriate factors, specifically those targeting differentiation process, is of great importance. In this study, w...
متن کاملHigh-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system.
Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.
متن کامل